Dr Morwenna Mckenzie, Dr Martin Wilkes WQR0035
Written evidence from Dr Morwenna Mckenzie (Postdoctoral researcher at Coventry University) and Dr Martin Wilkes (Assistant Professor at Coventry University)
Coventry University’s Centre for Agroecology and Water Resilience (CAWR) is a multidisciplinary research centre driving new understanding of resilient and socially just food and water systems internationally.
Response to relevant inquiry questions
1.1. Biological monitoring is central to current practice in river quality assessment worldwide, as exemplified by ecological status classification under the EU Water Framework Directive (WFD). Under this “biomonitoring” paradigm, certain groups of organisms are used as indicators of the pressures on waterbodies. The rationale for this approach is that, throughout their life cycles, resident organisms integrate the effects of pressures which might otherwise be difficult to detect through direct spot sampling of the abiotic environment.
1.2. Despite the importance of biomonitoring to river quality assessment, numerous direct measurements of the abiotic environment (e.g. pesticides, heavy metals, water discharge) are still collected for a range of purposes. Together, the collection of biological and environmental data should allow us to paint a detailed picture of river quality. Indeed, biological, chemical, geomorphological and hydrological processes are inextricably linked and cannot be fully understood in isolation. However, methods used to assess these different, but interdependent, aspects of river environments have developed independently of one another. Monitoring networks for different groups of organisms, and different aspects of the environment, do not overlap in time and space, hampering more complete characterisations of river health. Existing monitoring networks also suffer from extreme spatial bias, neglecting headwater streams which make up the vast majority of freshwater surface area and play a key role as greenhouse gas emitters - contributing over 70% of global riverine CO2, CH4, and N2O emissions.
1.3. Rivers are dynamic and hierarchical ecosystems, and their biological communities are highly variable with local responses to pressures set against a backdrop of changes occurring at nested scales throughout the river network. For example, changes in spilling regimes at wastewater treatment plants are superimposed on climate change (global), land-use change (river basin) and flood management impacts (river reach scale). Therefore, management tools that target fixed reference (“near-natural”) conditions and assume independence between observations through time and space (e.g. “RIVPACS”, the system underlying invertebrate biomonitoring in the UK), do not represent the multitude of drivers re-shaping biological communities. Originally developed to monitor nutrient pollution from agriculture and urbanisation, these tools are now widely used to indicate general river health. They lack the capacity to diagnose 21st Century pressures, such as global heating, extreme events, microplastics, pharmaceuticals and personal care products.
1.4. The pressures impacting rivers today are manifold, operating over a range spatial and temporal scales, and frequently interacting to amplify or dampen the effects of other pressures. For example, the problem of fine sediment (particles <2 mm in diameter) has become a major focus for river managers in recent years. Entering rivers from urban and intensive agricultural sources, fine sediment has pervasive impacts on river environments: smothering reproductive habitats of fish populations already impacted by global heating, increasing flood risk in concert with extreme events, and acting as a vector for numerous pollutants, among other negative effects1. Independent studies have repeatedly shown that the performance of biomonitoring tools designed to indicate fine sediment pressures is limited compared to those targeting other pressures,. In Europe, the vast majority (87%) of available biomonitoring methods target nutrient pollution. Of the relatively few methods which target other pressures, even fewer are demonstrably adequate indicators of those pressures. For instance, only eight of 90 available methods targeting a wide range of toxic substances (e.g. heavy metals, persistent organic pollutants) in European waters have been demonstrated to actually respond to the presence of the targeted substances7.
1.5. Ecological status classification, as set out by the WFD, appears to be relatively insensitive to environmental change and unresponsive to management intervention,. Furthermore, WFD classification focuses solely on the structure of river ecosystems (e.g. the range of species present), yet the first priority for managers should be to ensure functional integrity (e.g. organic matter processing, drinking water provision, fisheries production). Trait-based approaches, which focus on the effects that organisms have on their environment rather than on species’ names, have often been proposed as an alternative. However, the link between species’ traits and ecosystem functioning is weak and statistical issues obscure quantitative links between trait diversity and environmental change.
1.6. Monitoring has traditionally focused on groups of organisms that may be identified and counted in the field or under a microscope, such as fish, invertebrates and diatoms (single-celled algae). However, a stronger mechanistic basis for assessing river quality can be found through targeting microbial communities. The mainstreaming of molecular (e.g. DNA- and RNA-based) technologies now makes this feasible, and more cost-effective than traditional methods when applied at scale. In particular, monitoring the frequency of particular genes activated by bacteria and fungi in response to the occurrence of specific pollutants can provide near instantaneous diagnostic capability and a represents a direct measure of ecosystem functioning. UK authorities are slowly embracing molecular technologies but their focus has been on replacing techniques used to monitor the same target organisms (e.g. diatoms) within the existing monitoring framework, rather than seeking new and improved applications.
1.7. In summary, existing frameworks for monitoring and assessing river quality are firmly based upon 20th century problems, ideas and technologies. The steps required to update these frameworks in light of new environmental issues and emerging technologies all require a greater focus to be placed on data management, statistical literacy and high-performance computing. This includes statistical models that can correct for spatial bias in monitoring networks, bioinformatics protocols to process the outcomes of DNA- and RNA- based monitoring, artificial intelligence techniques trained to detect early warning signals, and process-based computer models simulating flows of water, pollutants and organisms within river networks.
1.8. A better approach to assessing river quality in the 21st Century lies in:
 Wilkes, M.A., Gittins, J.R., Mathers, K.L., Mason, R., Casas‐Mulet, R., Vanzo, D., Mckenzie, M., Murray‐Bligh, J., England, J., Gurnell, A. and Jones, J.I., 2019. Physical and biological controls on fine sediment transport and storage in rivers. Wiley Interdisciplinary Reviews: Water, 6(2), p.e1331.
 Li, M., Peng, C., Zhang, K., Xu, L., Wang, J., Yang, Y., Li, P., Liu, Z. and He, N., 2020. Headwater stream ecosystem: an important source of greenhouse gases to the atmosphere. Water Research, 190, p.116738.
 Poikane, S., Herrero, F.S., Kelly, M.G., Borja, A., Birk, S. and van de Bund, W., 2020. European aquatic ecological assessment methods: A critical review of their sensitivity to key pressures. Science of The Total Environment, p.140075.
 Birk, S., Chapman, D., Carvalho, L., Spears, B.M., Andersen, H.E., Argillier, C., Auer, S., Baattrup-Pedersen, A., Banin, L., Beklioğlu, M. and Bondar-Kunze, E., 2020. Impacts of multiple stressors on freshwater biota across spatial scales and ecosystems. Nature Ecology & Evolution, 4(8), pp.1060-1068.
 Wilkes, M.A., Mckenzie, M., Murphy, J.F. and Chadd, R.P., 2017. Assessing the mechanistic basis for fine sediment biomonitoring: Inconsistencies among the literature, traits and indices. River Research and Applications, 33(10), pp.1618-1629.
 Mckenzie, M., 2020. Quantifying the response of macroinvertebrates to gradients of fine sediment pollution. PhD Thesis (unpublished), Coventry University.
 Poikane, S., Herrero, F.S., Kelly, M.G., Borja, A., Birk, S. and van de Bund, W., 2020. European aquatic ecological assessment methods: A critical review of their sensitivity to key pressures. Science of The Total Environment, 740, p.140075.
 Haase, P., Hering, D., Jähnig, S.C., Lorenz, A.W. and Sundermann, A., 2013. The impact of hydromorphological restoration on river ecological status: a comparison of fish, benthic invertebrates, and macrophytes. Hydrobiologia, 704(1), pp.475-488.
 England, J. and Wilkes, M.A., 2018. Does river restoration work? Taxonomic and functional trajectories at two restoration schemes. Science of The Total Environment, 618, pp.961-970.
 Wilkes, M.A., Edwards, F., Jones, J.I., Murphy, J.F., England, J., Friberg, N., Hering, D., Poff, N.L., Usseglio‐Polatera, P., Verberk, W.C. and Webb, J., 2020. Trait‐based ecology at large scales: Assessing functional trait correlations, phylogenetic constraints and spatial variability using open data. Global Change Biology, 26(12), pp.7255-7267.
 Thompson, M.S., Bankier, C., Bell, T., Dumbrell, A.J., Gray, C., Ledger, M.E., Lehmann, K., McKew, B.A., Sayer, C.D., Shelley, F. and Trimmer, M., 2016. Gene‐to‐ecosystem impacts of a catastrophic pesticide spill: testing a multilevel bioassessment approach in a river ecosystem. Freshwater Biology, 61(12), pp.2037-2050.